Relationship between quantity of sequence data (expressed as expected read depth) and assembly quality (number of contigs and N50) for subsamples of reads.

<p>A) <i>De novo</i> assemblies of 75×2 bp paired end reads (insert size 150–300 bp). B) Scaffolding of contigs using 38×2 bp mate-pair reads (insert size 4–6 kb). Three random subsets of reads were analyzed for each coverage level except the highest, which used all of the reads.