CAR deficiency in mouse embryos after heart development causes subcutaneous edema, hemorrhage and lethality.

<p>(A) Western blot analysis of CAR protein levels in E14.5 and E15.5 embryos from <i>F/F;Cre</i> (cKO, n = 3) and littermate <i>F/F</i> control (ctrl, n = 3) animals from two litters after administration of tamoxifen at E12.5. Primary CAR antibody was RP 291. An antibody against calnexin was used as a loading control. (B) Macroscopic images of E16.5 embryos given tamoxifen at E12.5 showing subcutaneous edema and hemorrhage in cKO (n = 12) embryos but not in littermate <i>F/F</i> (n = 18) control embryos (ctrl), which appeared normal. Embryos were from 5 litters. Images were taken with a 4× objective. (C) Histological analysis of transverse sections of E16.5 embryos given tamoxifen at E12.5 showing subcutaneous edema in tamoxifen-treated cKO (n = 3) embryos while the littermate <i>F/F</i> controls (ctrl, n = 3) were normal. Embryos were from 3 litters. Images were taken with a 20× objective. Scale bar = 50 µm.