Lymphatic vessels of CAR deficient mouse embryos are structurally abnormal.

<p>(A, C) Confocal immunofluorescence images of whole-mount skin preparations from cKO (n = 5) and littermate <i>F/F</i> controls (ctrl, n = 4) at E16.5 co-stained with the antibodies rabbit anti-LYVE-1 (green) and hamster anti-CD31 (red) to visualize lymphatic vessels (A) and blood vessels (C), respectively. Embryos were from 2 litters. Scale bar = 50 µm. (B, D) Measure of vessel diameter. Data is presented as means ± SEM with at least four to six mice per group. (A) Immunofluorescence images showing dilated lymphatic vessels in cKO compared to ctrl embryos. (B) Bar graph showing significantly increased average diameter of subcutaneous lymphatic vessels in cKO versus <i>F/F</i> control (ctrl) embryos at E16.5. P<0.001. (C) Immunofluorescence images showing no differences in blood vessels in cKO compared to ctrl embryos. (D) Bar graph showing no significant differences in average diameter of subcutaneous blood vessels in cKO versus <i>F/F</i> control (ctrl) embryos at E16.5. P = 0.76.