CAR is expressed in lymphatic endothelial cells during mouse development and localizes to cell-cell junctions.

<p>Confocal images of whole-mount skin preparations from wildtype (A, B) (n = 3) and cKO (C) (n = 3) E16.5 embryos stained by immunofluorescence with the antibodies rabbit anti-CAR IG1 (green), hamster anti CD31 (red) and rat anti LYVE-1 (purple) to visualize CAR expression in blood vessels (CD31highLYVE-1neg) and lymphatic vessels (CD31lowLYVE-1pos). Animals were from 3 different litters (A) Low magnification images showing CAR expression in lymphatic vessels but not in blood vessels. Scale bar = 50 µm (B). High-magnification images showing distribution and partial co-localization of CAR with CD31, and LYVE-1 at lymphatic endothelial cell-cell junctions (arrows). Scale bar = 50 µm. (C) Low magnification images showing efficient downregulation of CAR in lymphatic vessels of cKO embryos. Scale bar = 50 µm.