Endogenous and exogenous paxillin, Hic-5 and vinculin colocalize in integrin-mediated adhesions of varying size.

<p>(A) Immunofluorescence images of NIH 3T3 cells spread on 2D fibronectin for 4hrs and stained for paxillin (top panels), Hic-5 (middle panels) and vinculin (bottom panels). Masks indicate that the endogenous proteins localize to all adhesions (<1 μm² to >10 μm²). Blue line indicates the cell edge as determined by F-actin staining. Representative images of cells co-expressing (B) mRFP-paxillin and (C) mRFP-Hic-5 with vinculin-YFP. Line profiles indicate fluorophore-tagged protein colocalization in all adhesion structures. (D) Representative image and line profiles of mRFP-paxillin and zyxin-YFP colocalization in adhesions. (E) Average Pearson's Correlation analyses indicate a high level of colocalization of exogenous proteins. Error bars are standard errors of the mean.