Vinculin preferentially interacts with Hic-5 rather than paxillin in 3D adhesion contacts.

<p>(A) Images of NIH 3T3 cells spread on 2D fibronectin (FN) or 3D cell-derived matrix (CDM) for 4hrs stained for paxillin/Hic-5 (green), fibronectin (blue) and F-actin (red). (B) Average Pearson's Correlation analyses of mRFP-paxillin or mRFP-Hic-5 with vinculin-YFP in 3D adhesions reveals no significant difference in colocalization. (C) Quantitation and representative images of (D) mRFP-paxillin and (E) mRFP-Hic-5 FRET efficiency with vinculin-YFP in 3D adhesions demonstrating FRET between vinculin-YFP and mRFP-Hic-5 but not mRFP-paxillin in 3D adhesions. Error bars are standard error of the mean and are calculated from a minimum of 15 cells from 3 individual experiments.