Colocalization of eIF4A1 and Paxillin.

<p>(A) The localization of eIF4A1 and paxillin in the EC was determined by confocal microscopy. Cell were treated and prepared for staining and microscopy. The scale bar is equal to 10 µm. Data is representative of 4 independent experiments. (B) eIF4A1 and paxillin colocalization was determined by the ImageJ plugin Colocalization Finder. Mander's Overlap coefficients were 0.977 (mIgG), 0.929 (HLA class I), 0.933 (thrombin) and 0.888 (bFGF). The data represents 4 independent experiments. (C) Intensities of the colocalization of 3 images per group were determined for each independent experiment (Avg ± SD): mIgG (14.3±4.3), HLA class I (30.2±4.1), thrombin (19.1±7.8) and bFGF (11.7±3.0). The colocalization intensity of eIF4A1 and paxillin in the HLA class I treated group was significantly increased compared to the unstimulated and bFGF groups; mIgG (p = 0.01), thrombin (p = 0.09) and bFGF (p = 0.003) as determined by student t-test.