Reduction of LDLR expression in DJ-1-knockout cells and in DJ-1-knockout mice.

<p>A. Mouse liver cells from wild-type and DJ-1-knockout mice were immunostained with anti-LDLR and anti-DJ-1 antibodies. The cells were then reacted with an FITC-conjugated anti-rabbit IgG or with a rhodamine-conjugated anti-rabbit IgG for 1 hr, and their nuclei were stained with DAPI. The cells were then observed under a fluorescent microscope as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038144#s4" target="_blank">Materials and methods</a>. B. Liver sections from wild-type and DJ-1-knockout mice were immunostained with anti-LDLR and anti-DJ-1 antibodies and visualized after reaction with an FITC-conjugated anti-rabbit IgG as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038144#s4" target="_blank">Materials and methods</a>. Nuclei were also stained with DAPI.