LPS-induced up-regulation of IFN-γ and I-TAC is enhanced in DJ-1 knockout mice.

<p>Levels of IFN-γ and I-TAC in substantia nigra (SN) were up-regulated by local injection with LPS (1 μg /1 μl) in DJ-1 KO mice. (A) Immunohistochemical staining of IFN-γ in substantia nigra of WT and DJ-1 KO mice Day-5 after injection of LPS. (B) Quantitative results (ELISA) show the increase of IFN-γ expression in substantia nigra of DJ-1 KO mice. (C) Immunohistochemical staining of I-TAC in substantia nigra of WT and DJ-1 KO mice Day-5 after injection of LPS. (D) Quantitative results (ELISA) show the up-regulation of I-TAC expression in substantia nigra of DJ-1 KO mice. The typical stained-cells were indicated by arrows. Data were normalized as percentage of the mean of basal expressional levels in WT mice (con) and presented as mean ± S.E.M. (n = 4–5 for each group) * p<0.05. SNpc: substantia nigra pars compacta; SNr: substantia nigra pars reticulate. Scale bar: 0.2 mm.