Reduction of <i>LDLR</i> gene expression in DJ-1-knockdown cells and DJ-1-knockout mice.

<p>A. Relative mRNA levels of LDLR were examined by quantitative RT-PCR (real-time PCR) in NIH3T3 and its DJ-1-knockdown D2 cells, in livers from wild-type and DJ-1-knockout mice at 25 and 36 weeks of age and in the established liver cell line from wild-type and DJ-1-knockout mice. Actin or GAPDA mRNA was also amplified by real-time PCR as loading controls. Statistical analyses were carried out using Student’s <i>t</i>-test. Number of experiments (n) is 3. B and C. Proteins extracted from NIH3T3 and D2 cells (B) and from livers of wild-type and DJ-1-knockout mice at 13 and 51 weeks of age (right panels in C) were analyzed by Western blotting with anti-LDLR, anti-DJ-1 and β-actin antibodies. β-actin was used as a loading control. Proteins from the liver of wild-type mouse at 25 weeks of age were analyzed by Western blotting with an anti-LDLR antibody (left panel in C).