Thermal denaturation curves of p53CTD L344A in the presence of peptides from the array.

<p>20 µM protein was heated from 25°C to 65°C with or without 100 µM peptide. The data were fit to a sigmoidal curve describing a transition between two states. Raw data for two repeats (squares and hollow circles) are shown in the background. The dashed lines represent the averaged melting temperatures for both repeats.