Far-UV CD spectra of the PfN–PCoil and PfN fragments after purification by SEC:

<p>(<b>A</b>) <b>PfN and PfN–PCoil at 20°C;</b> (<b>B</b>) <b>PfN–PCoil at 20°C and 60°C;</b> (<b>C</b>) <b>PfN at 20°C and 60°C.</b> In all panels the vertical axis measures mean residue ellipticity Θ in degrees cm² dmol^-1. The CD data was collected between 195 and 260 nm, with protein concentrations of 0.2 mg/ml (PfN–PCoil) or 0.3 mg/ml (PfN), in 20 mM phosphate buffer (Na₂HPO_4/NaH₂PO₄), 100 mM NaCl, pH 7.4. Measurements were taken in a 0.5 mm path length cell.