Thermal stability of PhaF.

<p>(<b>A</b>) Far-UV CD spectra of PhaF at different temperatures. Recovered spectra were registered upon immediate cooling down the scanned sample and after a 30 min waiting period at 20 °C. (<b>B</b>) Near-UV CD spectra of PhaF. (<b>C</b>) Far-UV CD spectra of the isolated C-terminal domain of PhaF (C-PhaF protein). <b><i>Inset</i></b>, difference CD spectrum of C-PhaF (20°C–90 °C). (<b>D</b>) CD-monitored temperature scans of PhaF. Solid line indicates fitting of the far-UV CD-monitored transition (protein concentration: 3.8 µM) to the Gibbs-Helmholtz equation (see Materials and Methods). <b><i>Inset</i></b>, temperature scan of C-PhaF monitored at 205 nm.