Suppression of Tap42 expression in wing imaginal discs interrupts HH signaling, hampers mitosis, and triggers apoptosis.

<p>Panel A: Isolated wing imaginal discs were immunostained with antibodies recognizing Tap42 (green) and multiple components in the HH signaling pathway, including Ptc, Smo, and Ci (red). Control wing discs displayed strong Tap42 (A1) expression and the expected expression pattern for Ptc (B1), Smo (C1), and Ci (D1). Suppression of <i>Tap42</i> with the <i>pnr-Gal4</i> or ap<i>-Gal4</i> driver effectively reduced Tap42 levels in wing discs (A2 & A3). While the levels of the HH receptor Ptc were unaffected by Tap42 silencing (B3), the expression of other downstream components of HH signaling, Smo (C3) and Ci (D3), were abrogated. Suppression of Tap42 with the <i>pnr-Gal4</i> driver did not alter the expression pattern of HH signaling as shown in B2 (Ptc), C2 (Smo) and D2 (Ci). Genotypes: (A1, B1, C1, & D1) <i>UAS-Tap42^RNAi/+</i> as control. (A2, B2, C2, & D2) <i>UAS-Tap42^RNAi/+; pnr-Gal4/+</i>. (A3, B3, C3, & D3) <i>ap-Gal4/UAS-Tap42^RNAi; +/+</i>.