Signaling properties of Lpp-associated Hh and Hh-N*.

<p>(A) Cartoon depicting the fat body to wing disc signaling assay of secreted Hh. (B) IF of wing discs from larvae secreting Hh from the fat body, and Lpp RNAi larvae, stained for Hh, Ci₁₅₅, and Lpp. Scale bar = 50 µm. In all wing discs, A denotes the anterior compartment, P the posterior compartment; yellow lines indicate the compartment boundary. Scale bar = 50 µm. (C and D) Quantification of (C) Hh and (D) Ci₁₅₅ staining of wing discs shown in (B). Translucent lines indicate ±SD (<i>n</i> = 12). (E) Phosphorylation status of Fused in wing discs of larvae secreting Hh from the fat body and Lpp RNAi larvae, analyzed by WB. (F) Ci₇₅ repressor levels in wing discs of larvae secreting Hh from the fat body, and Lpp RNAi larvae, analyzed by WB. (G) IF of wing discs from larvae secreting Hh or Hh-N* from the fat body, stained for <i>dpp</i>LacZ and, to mark cell boundaries, with phalloidin. Hh-N* was generated by expressing Hh in the fat body of Lpp RNAi animals. Scale bar = 50 µm. (H) Wing disc anterior to posterior compartment ratio of larvae secreting Hh or Hh-N* from the fat body and Lpp RNAi larvae. Error bars indicate ± SEM (<i>n</i> = 20). ***<i>p</i><0.0005; ****<i>p</i><0.00005.