Fitness data for all significantly affected sensitive strains identified from this study were mapped onto the <i>S. cerevisiae</i> BioGRID interaction dataset using Cytoscape.

<p>The fitness scores (the difference in the mean of the log₂ hybridization signal between LZM+1 µM ZnCl₂ and LZM+100 µM ZnCl₂) of these sensitive strains were then used to identify and create a smaller sub-network (283 genes) containing the sensitive genes and the non-sensitive and essential genes that link them through known genetic and physical interactions. The sub-network was then assessed for significant overrepresentation of Gene Ontology (GO) Cellular Component categories. These categories were visualized as a linked network. Node color of categories indicates the significance of representation (white = not identified as significant) and node size indicates the number of genes identified present in each category. Edge arrows indicate hierarchy of GO terms. For clarity, only GO Cellular Component categories with a p-value<0.0005 are shown. A separate GO enrichment assessment identified overrepresentation of all GO categories in the sub-network. This analysis was used to generate visual representations of the GO processes and cellular components identified showing the genes involved in these processes. In these cases, node color indicates the sensitivity of each deletion strain in our study (fitness score). The edge color defines the interaction type between nodes (from the BioGRID database).