Genetic interaction network of TARGET screen hits.

<p>Genetic and biochemical interactions of the 46 genes identified in the TARGET screen were mined from the Drosophila Interactions Database (<a href="http://www.droidb.org" target="_blank">www.droidb.org</a>). Lines linking each gene indicate direct genetic or protein-based interactions. Reflexive loops indicate a direct genetic or protein-based interaction with itself. Genes were then organized into functional groups based on GO term analysis. Each gene in the network was colour-coded based on the severity of the climbing phenotype caused by RNAi-mediated gene knockdown (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099362#pone-0099362-g005" target="_blank">Figure 5</a>). Red indicates a “Severe” phenotype; orange indicates a “Intermediate” phenotype; and blue indicates a “Weak” phenotype. The gene <i>rhea</i> is coloured gray as it was not part of this screen but was previously identified, using the same approach, to be required for muscle maintenance. Note cluster of genes that caused “Severe” or “Intermediate” phenotypes in the ‘Sarcomeric’ group.