Dose-dependent response to rosuvastatin in cell migration of VSMC exposed to LDL.

<p>Bar diagram for (<b>A</b>) dose dependent effect of rosuvastatin (2.5–40 µM) in wound regeneration by VSMC treated without/with agLDL 8 hours after wounding. P<0.01 (Kruskal-Wallis test) for rosuvastatin dose-response effect and P<0.001 for simvastatin effect (Man-Whitney test) vs agLDL-group. (<b>B and C</b>) VSMC were treated without or with rosuvastatin (10 µM) since 2 hours before (2 h-pre LDL) or 8 hours after (8 h-post LDL) adding agLDL to the cell culture medium. <b>B:</b> staining with Herxheimer reagent (Sudan III and Sudan IV) (red colour) as a control for intra-cellular accumulation of lipids. Ø refers to agLDL-VSMC non treated with rosuvastatin. <b>C:</b> Results shown as mean±SEM (% of wounded area at time 0) refer % of the wound regenerated area 4 and 8 hours after wounding (n=4 independent experiments); * p<0.05 compared to the agLDL-group (Man-Whitney test).