AMPK activity in response to IL-6 in myocytes from healthy and people with type 2 diabetes.

<p>Muscle precursor cells derived from the vastus lateralis muscle of healthy (He) (n = 5), and obese people with type 2 diabetes (DM) (n = 5) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039657#pone-0039657-t003" target="_blank">Table 3</a>), were differentiated into myocytes and were treated with recombinant IL-6 for 30 or 60 minutes. Controls (Ctrl) were treated with PBS for 60 minutes. Protein was isolated and AMPK activity was measured (A) AMPK α1 activity in response to IL-6 was compared between groups by a two-way ANOVA. (B) AMPK α2 activity in response to IL-6 was compared between groups by a two-way ANOVA. While there was no effect of group, there was an interaction (P<0.01) and thus Bonferroni post-tests were performed. Effect of treatment within groups was performed by one-way ANOVAs. Data are mean ± SE. *Results from Bonferroni post-tests, relative to He myocytes; *P<0.05, **P<0.01, ***P<0.001.