IL-6Rα protein expression in skeletal muscle tissue biopsies and in He, Ob and DM myocytes in response to IL-6.

<p>(A) Protein expression of IL-6Rα was assessed in skeletal muscle biopsies from the vastus lateralis muscle of non-obese (n = 10) or obese (n = 10) normal glucose tolerant (NGT) subjects and non-obese (n = 10) or obese (n = 9) subjects with type 2 diabetes (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039657#pone-0039657-t001" target="_blank">Table 1</a>), using western blot analysis. Due to the variability in these <i>in vivo</i> samples, all blots are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039657#pone.0039657.s001" target="_blank">Figure S1</a>. A two-way ANOVA was performed, comparing the effect of obesity and the effect of diabetes on IL-6Rα protein expression. (B) Protein expression of IL-6Rα was assessed in satellite cells isolated from healthy (He) (n = 7), obese (Ob) (n = 7) and obese people (n = 7) with type 2 diabetes (DM) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039657#pone-0039657-t002" target="_blank">Table 2</a>). Satellite cells were differentiated into myocytes and treated with IL-6 (100 ng/ml) or control (PBS) for 30, 60 and 120 min. (C) Baseline IL-6Rα protein expression was normalized to total protein expression (as determined by reactive brown staining) and compared between groups by two-way ANOVAs. (D) IL-6 induced IL-6Rα protein expression was normalized to total protein and compared between groups by two-way ANOVAs and effect of time within groups was assessed with one-way ANOVAs. Data are presented as fold change (FC) between the control samples presented in B and C and samples treated with IL-6 (100 ng/ml). Data are mean ± SE. Groups were compared in pairs (i.e. He vs Ob, He vs DM and Ob vs DM) by two-way ANOVAs. Results from ANOVAs are marked in the figures with connecting capped arcs. *Results from Bonferroni post-tests, relative to He myocytes; *P<0.05, **P<0.01, ***P<0.001.