Decreased energy intake in Het GIP Tg mice.

<p>After 11–12 weeks of LF or HF diet feeding, WT and Het GIP Tg mice (<i>n</i> = 4∼5/group) were placed in individual cages, and energy intake was assessed during the light and dark cycle. <b>A and B.</b> Cumulative energy intake in LF (A)- and HF (B) diet-fed Het GIP Tg and WT littermates. <b>C–D.</b> Histological analyses of brain tissue from Het GIP Tg and WT littermates following 13 weeks of LF and HF feedings. <b>C.</b> Representative images of GIP immunoreactivity in the hypothalamus. Brain sections were stained with GIP (green), PACAP (red) and DAPI (blue). The scale bar indicates 100 µm. <b>D.</b> Percentage of positive cells. GIP-, PACAP-, and GIP/PACAP double positive cells were expressed as the percentage of positive cells, respectively. All data represent the mean ± S.E.M. and significance was tested using ANOVA with a Newman-Keuls post hoc test, where ** represents p<0.05 <i>vs</i> indicated group; <i>NS</i> represents not significant.