MR mice on HFD have lower body weight gain despite increased energy intake.

<p>(<b>A</b>) Cumulative food intake was measured on HFD mice twice a week for 99 days. (<b>B</b>) Body weights (BW) were measured on HFD mice twice a week for 99 days. (<b>C</b>) Body weight gain was the difference between the weights at the beginning and at the end of the study. (<b>D</b>) Energy intake was calculated based on the average daily energy (kcal) intake per gram body weight. (<b>E</b>) Absorption efficiency was estimated based on the amount of food intake and fecal output within a 24 h period as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051357#s2" target="_blank">Methods</a> section. Data is presented as the mean ± SD of 8 mice per treatment group and analyzed by Two-way ANOVA followed by Bonferroni post-tests (A and B) or Student’s unpaired <i>t</i>-test (C–E). *p<0.05, ***p<0.001.