IgG1:IgG2a ratios of HIV p24Gag-specific antibodies in sera of uninfected and <i>Mtb</i>-infected mice vaccinated with MultiHIV DNA/protein.

<p>Uninfected C57BL/6 mice, or mice infected with <i>Mtb</i> via the respiratory route, were immunized and boosted with MultiHIV DNA/protein, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041205#s4" target="_blank">Materials and Methods</a>. IgG1:IgG2a ratios were calculated using p24Gag-specific antibody titers determined with ELISA in sera 4 wk post-vaccination. Median IgG1:IgG2a ratio of 6–8 mice/group from one individual experiment is shown as a solid line. The box defines the 75th and 25th percentiles and the whiskers define the maximum and minimum values (**: P<0.01; ***: P<0.001). The IgG1:IgG2a ratios were determined in two separate experiments.