Effect of A2bAR elimination on tissue insulin signaling post HFD.

<p>Western blot analysis of different insulin responsive tissues; one representative WT and A2bAR KO pair is shown. <b>A.</b> phospho-Thr308 Akt (60 kDa), phospho-Ser473 Akt (60 kDa), total Akt (60 kDa) and β-actin (43 kDa) in liver at base line, and in visceral fat tissue and gastrocnemius tissue 15 minutes post-insulin injection (1 U/kg Humulin R ). <b>B.</b> Tissue A2bAR mRNA levels were measured by qPCR. Twelve-week-old WT mice (C57BL/6J) were analyzed and compared to similar mice subjected to additional 16 weeks of HFD. The expression of the receptor is relative to the baseline value, measured in 12 week old mice before the 16 weeks diet (denoted as HFD), and normalized to 18 S rRNA (denoted as 18 S), p-value = 0.0087 for liver; p-value = 0.0073 for visceral fat; p-value = 0.0009 for gastrocnemious muscle.