Expression levels of insulin signaling pathway-related proteins were reduced in livers of HFD-fed DKO mice compared with WT mice.

(A) Protein levels of insulin signaling pathway-related molecules in livers of CD-fed (3 mice each) or HFD-fed (4 mice each) mice were analyzed by immunoblotting. (B) Relative expression levels are presented by measuring band intensities after correction with those of β-Actin. Open and closed bars indicate WT and DKO mice, respectively. Data are expressed as means ± S.D. *P#P##P<0.01 as compared between CD and HFD groups.