Effect of CD68-driven expression of A2bAR on tissue insulin signaling.

<p>Western blot analysis of liver and visceral fat derived from matching WT, CD68-Tg and A2bAR KO mice post 16 weeks of HFD and 15 minutes following insulin injection. <b>A</b>. Levels of phospho-308 Akt (p308 Akt, 60 kDa), phospho-473 Akt (p473 Akt, 60 kDa), and total Akt (60 kDa) were probed by Western blot analysis, using β-actin (43 kDa) as loading control. Shown are representative out of 3 sets. <b>B</b>. Quantification of Western Blot results was performed with Image J software (<a href="http://rsb.info.nih.gov/ij/" target="_blank">http://rsb.info.nih.gov/ij/</a>). Protein levels were normalized to total Akt and β-actin. Data are averages ± SD. *Student two-tail t-test assuming equal variance was found significant only when p<0.05. WT to A2bAR KO: Liver p308 Akt p-value = 0.0007, p473 Akt p-value = 0.0219; Visceral fat: p308 Akt p-value = 0.0340, p473 Akt p-value = 0.0395; CD68-Tg to A2bAR KO: Liver p308 Akt p-value = 0.0327, p473 Akt p-value = 0.0349; Visceral fat: p308 Akt p-value = 0.1054; p473 Akt p-value = 0.0221.