Standard curve of miR-21, miR-223, miR-218 and miR-25 using synthetic miRNAs.
- Bo-sheng Li (146369)
- Yong-liang Zhao (146374)
- Gang Guo (146383)
- Wei Li (7081)
- En-dong Zhu (146391)
- Xiao Luo (146399)
- Xu-hu Mao (146406)
- Quan-ming Zou (146412)
- Pei-wu Yu (146417)
- Qian-fei Zuo (146423)
- Na Li (6550)
- Bin Tang (56654)
- Kai-yun Liu (146430)
- Bin Xiao (146435)
Publication date
July 2012
DOIAbstract
<p>Ten-fold serial dilution of synthetic miRNA was used to generate the standard curves. Linearity was confirmed within these concentrations for miR-21, miR-223, miR-218 and miR-25 ranging from 0.1 to 0.000001 fmol. (<i>miR-21</i>: y = −3.337x+14.77 (R<sup>2</sup> = 0.999); <i>miR-223</i>: y = −3.121x+17.37 (R<sup>2</sup> = 0.997); <i>miR-218</i>: y = −3.071x+17.79 (R<sup>2</sup> = 0.998); <i>miR-25</i>:y = −3.114x+17.47 (R<sup>2</sup> = 0.993)).</p
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