Relative levels of miR-17∼92a, miR-106b∼25, and miR-106a∼363 cluster components in EWS cells and hMPCs.

<p>Relative levels of the individual miRs within a given cluster in each cell line were determined by qRT-PCR using a best-fit linear equation generated by the amplicon standard curve in order to account for differences in primer efficiency. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063032#pone.0063032-Young1" target="_blank">[57]</a> Values represent the mean and standard deviation of biological triplicates.