miRNAs DE by cell type.

<p>(A) The 10 most stable miRNAs across all cell types are shown for both NormFinder (left) and Coefficient of Variation (CV) methods (right). (B) Validation of NanoString-derived data for <i>miR-301a-3p</i>by qRT-PCR data. The X-axis represents the expression level of each miRNA normalized to <i>miR-30c-3p</i> using 2^−ΔCt method. Each point represents the mean ± SEM of 5 subjects for each cell type. (C,D) Validation of microRNAs DE by cell type. The NanoString-derived data for the indicated miRNAs was validated by qRT-PCR using RNA isolated from the 5 hematopoietic cell types from two different preparations of cells and RNA. qRT-PCR data was normalized and presented as in Figure 4B.P, platelets; T, T-cells; B, B-cells; G, granulocytes; E, erythrocytes.