Immunofluorescence analysis of cell surface mERα in rat hippocampal neurons.

<p>mERα expression (green fluorescence) in unpermeated hippocampal neuronal cells and MAP2 expression (red fluorescence) in Triton X100 permeated hippocampal neuronal cells: (<b>A</b>) untreated, (<b>B, C</b>) CMP treated or (<b>D</b>) NAC exposed before CMP treatment. Note the yellow spots in (<b>B</b>) and (<b>C</b>) suggesting a colocalization of ERα and MAP2 in soma (<b>B</b>) and dendritic protrusions (<b>C</b>) of CMP exposed cells. No yellow spots are instead observable in untreated and NAC/CMP exposed hippocampal cells. Cells were counterstained with Hoechst dye to reveal nuclei (blue staining). Magnifications: (<b>A</b>) (<b>B</b>) (<b>D</b>) 3500×; (<b>C</b>) 6000×.