Synaptophysin-staining analysis in the hippocampus.

<p>Representative MosaiX montage images that covers all the CA3 and part (after trimming) of the CA1 hippocampal regions: in green is the Map-2 immunostaining and in red the synaptophysin staining in animals from the IC (A), SC (B), and WM (C) groups; the white bar represents 500 μm. (D) The bars represent the average synaptophysin/Map2 staining (±SEM) in each group (IC, SC, and WM) for each ROI (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132676#sec002" target="_blank">Methods</a>). Note that only in the CA3 <i>SOd</i> (<i>stratum oriens distal</i>), the CA3 <i>SLd</i> (<i>stratum lucidum distal</i>), and the CA1 <i>SO</i> (<i>stratum oriens</i>) were significant differences found between groups. Fisher <i>post hoc</i> analysis of the WM vs. the IC ***p<0.001, **p<0.01, *p<0.05; WM vs. the SC ##p<0.01.