Synaptophysin/Map2 segmentation and Map2 staining area analysis.

<p>(A) A representative synaptophysin (Red) / Map2 (Green) stained image is shown with the drawings that defined the different hippocampal dendritic segments regions of interest (hippocampal segments ROIs). These hippocampal segments ROIs included the CA3 <i>stratum oriens</i>, divided into 3 regions based on their proximity to the dentate gyrus (DG): <i>stratum oriens distal</i> (<i>SOd</i>), <i>stratum oriens medial</i> (<i>SOm</i>), and <i>stratum oriens proximal</i> (<i>SOp</i>); the CA3 <i>stratum lucidum</i> was also divided into 3 regions, the <i>stratum lucidum distal</i> (<i>SLd</i>), <i>stratum lucidum medial</i> (<i>SLm</i>), and <i>stratum lucidum proximal</i> (<i>SLp</i>). The CA3 <i>stratum radiatum</i> was divided into 6 regions, depending on their proximity to the DG and to the pyramidal cell soma: <i>stratum radiatum distal medial</i> (<i>SRdm</i>), <i>stratum radiatum medial medial</i> (<i>SRmm</i>), <i>stratum radiatum proximal medial</i> (<i>SRpm</i>), <i>stratum radiatum distal distal</i> (<i>SRdd</i>), <i>stratum radiatum medial distal</i> (<i>SRmd</i>), and <i>stratum radiatum proximal distal</i> (<i>SRpd</i>). Finally, the last 2 ROIs correspond to the CA1 <i>stratum oriens</i> (CA1 <i>SO</i>) and the CA1 <i>stratum radiatum</i> (CA1 <i>SR</i>). In the bar graph (B) the Map2-stained area expressed in pixels is shown for each hippocampal segment ROI. It is important to emphasise that No significant differences were found among groups (IC, SC, and WM) in the Map2-stained area used for the synaptophysin analysis.