Hippocampal neurons are differentiated and present distinct morphology for both the axon and dendrites.

<p>Dendrites have shorter process and taper more gradually (arrows), while axons display a long, narrow process with minimal tapering (arrows in all figures). (A–C) Expression of phosphorylated neurofilaments SMI-31 (red) and microtubule-associated protein MAP2 (green) (A) Double-label immunofluorescence of SMI-31 (red) and MAP2 (green) at 4 DIV (days <i>in vitro</i>). Dendrites (MAP2) are shorter with gradual tapering projections whereas axon (SMI-31) stain display long narrow processes. (B) Double-label immunofluorescence of SMI-31 (red) and MAP2 (green) at 7 DIV. (C) Double-label immunofluorescence of SMI-31 (red) and MAP2 (green) at 12 DIV. Bar is 50 µm.