FLNa-EGFP binds and internalizes CCR2B.

<p>M2 cells (first column left panel), A7 cells (second column left panel) and HEK293 cells were transfected with pcDNA3-FLAG-CCR2B. Central panel shows HEK293-CCR2B cells treated with 100 nM of FLNa siRNA. M2 cells were transfected with pcDNA3-FLAG-CCR2B and FLNa-EGFP (right panel). Cells were further incubated with CCL2 for the times indicated and stained for CCR2B (red). The images were deconvolved using a theoretical point spread function before analysis of colocalization. Colocalization is shown in white with Pearson coefficient expressed in %. Images are from one single layer of the Z stacks. White dotted lines show the contour of the cells. Experiments were done in duplicates and repeated three times in each experiment an average of 20 cells were analyzed. Scalebars (10 μm).