PCNA colocalizes with HLA I on the cell surface.

<p>DB and MCF-7 cells were first incubated with 2.5 µg anti-HLA I followed by staining with anti-mIgG-Dylight 594 (Red). Cells were then incubated with 2.5 µg anti-PCNA-Alexa Fluor 488 (Green). Cells were imaged on the Zeiss LSM 510 Confocal Laser Microscope using 40×, 1.2 NA, 0.28 WD (water), C-Apochromat objective utilizing 561 nm and 488 nm wavelengths at 43% and 57% transmittance respectively. Areas of colocalization can be seen as yellow when overlaid. (<b>A</b>) DB morphology. (<b>B</b>) DB cells were incubated with mIgG2a-Dylight 594 and mIgG2a-Alexa Fluor 488 and overlaid for background control. (<b>C</b>) HLA I expression on DB cells. (<b>D</b>) PCNA expression on DB cells. (<b>E</b>) Colocalization (Yellow) of HLA I and PCNA on the surface of DB cells. (<b>F</b>) Morphology of DB cell examined under 3× zoom. (<b>G</b>) HLA I expression on DB cell 3× zoom. (<b>H</b>) PCNA expression on DB cell 3× zoom. (<b>I</b>) Colocalization of HLA I and PCNA (Yellow) on DB cell 3× zoom. (<b>J</b>) Morphology of MCF 7 cells, 2× zoom. (<b>K</b>) MCF-7 cells were incubated with mIgG2a-Dylight 594 and mIgG2a-Alexa Fluor 488 and overlaid for background control. (<b>L</b>) HLA I expression on MCF-7 cells, 2× zoom. (<b>M</b>) PCNA expression on MCF-7 cells, 2× zoom. (<b>N</b>) HLA I and PCNA do not colocalize on the surface of MCF-7 cells, 2× zoom.