FLNa-EGFP colocalizes with actin at the membrane and in fibres.

<p>(<b>A</b>) Confocal images of FLNa, FLNa-EGFP and actin expression in cells after immunostaining. M2 cells transiently transfected with pcDNA3.1-FLNa-EGFP or pREP4-FLNa and untransfected A7 and M2 cells, were seeded on top of glass coverslips, fixed, permeabilized, incubated with anti-FLNa and goat anti-mouse Alexa Fluor 488 and stained with rhodamine-phalloidin before mounting. (<b>B</b>) Confocal images of FLNa-EGFP expression in M2 cells transiently transfected with pcDNA3.1-FLNa-EGFP. Cells were seeded on top of glass coverslips, fixed, permeabilized and incubated with anti-FLNa and Texas Red goat anti-mouse. All images are from one single layer of the Z stacks. The colocalization between (green) and (red) was analyzed using Imaris colocalization software and is shown in white. Images are representative of at least three independent experiments. Scalebars (10 μm).