Membrane dynamics of the GABA_AR γ2 and α5 subunits in relation with inhibitory synapses.

<p>(A, B) Examples of individual trajectories of QDs coupled to γ2 (A1) and α5 (B1) outside (black) or inside (grey) inhibitory synapses and their corresponding MSD (A2, B2) and instantaneous diffusion coefficients as a function of time (A3,B3). Scale, 0.5 µm (A1) and 1 µm (B1). Note the reduced diffusion and increased confinement of γ2 and α5 in inhibitory synapses <i>vs</i>. extrasynaptic membrane. (C–E) Diffusion behavior of receptor populations analyzed from pooled QD trajectories. Averaged MSD <i>vs</i>. time function (C), explored surface area (D, (median EA ±25%–75% Interquartile Range IQR; KS test ***p<10⁻³, **p<10⁻²), diffusion coefficient (E, median D ±25%–75% IQR; KS test ***p<10⁻³) of γ2 (black) and α5 (white) trajectories in inhibitory synapses (inh) and in extrasynaptic compartments (ex). (F) Dwell time (mean ± SEM) of the indicated receptors at inhibitory synapses (t-test, ***p<10⁻³).