Effects of gephyrin clustering interference on the diffusion properties of the GABA_AR γ2 subunit at inhibitory synapses.

<p>(A) Individual trajectories of QDs coupled to γ2 outside (black, ex) or inside (green, inh) inhibitory synapses in mRFP-gephyrin knock-in mice neurons transfected with GFP, venus-G(2) or venus-E constructs. Scale, 0.5 µm. (B) Over-expression of the venus-G(2) (black) or venus-E (grey) chimera did not change the diffusion coefficient (median D ±25%–75% IQR; KS test, ns: not significant) of γ2 outside (ex) or inside (inh) inhibitory synapses as compared to the respective control conditions (GFP, white). In contrast, the gephyrin dominant-negative venus-G(2) (black) and venus-E (grey) constructs increased the explored surface area (C, median EA ±25%–75% IQR; KS test, ns: not significant, ***: p<10⁻³), and decreased the dwell time (D, mean ± SEM; t test *: p<0.05) and the proportion of trapped γ2 (E, mean ± SEM; t test *: p<0.05) at inhibitory synapses.