Validation of microarray results for differentially regulated genes (DRGs).

<p>The confirmation of selected differentially regulated genes was achieved by quantitative real-time polymerase chain reaction (qRT-PCR) using total RNA from SK-N-MC cells: (<b>A</b>) treated with 5 nM rotenone for 4 weeks and 0 nM (vehicle-treated control); (<b>B</b>) treated with 50 nM rotenone for 4 weeks and 0 nM (control). Comparison of the qRT-PCR analysis results of rotenone-treated samples to vehicle-treated samples showed that all genes changes were significantly different (P<0.05). The qRT-PCR average fold changes of selected DRGs in the rotenone treated samples, normalized to <i>B2M</i> (beta-2-microglobulin) and relative to expression on the vehicle-treated cells (dotted red line) are shown (mean ± SEM; *P<0.05; t-test, qRT-PCR vs microarray result, n = 3). Pearson’s test found significant correlation between microarray and qRT-PCR data in the 4w5 nM group (r² = 0.9029, P<0.0001) and in the 4w50 nM group (r² = 0.726, P<0.0017). <i>PTPRC</i> (protein tyrosine phosphatase, receptor type, C), <i>HSPA1A</i> (heat shock 70 kDa protein 1A), <i>CLIC2</i> (Chloride intracellular channel 2), <i>SNCA</i> (α-synuclein), <i>IGFR1</i> (IGF1 receptor), <i>MAP2K4</i> (mitogen-activated protein kinase kinase 4 ), <i>SLC16A3</i> (solute carrier family 16, member 3 -monocarboxylic acid transporter), <i>LOXL1</i> (lysyl oxidase-like 1), <i>APOE</i> (Apolipoprotein E), <i>SELENBP1</i> (selenium binding protein 1), <i>DDIT3</i> (DNA-damage-inducible transcript 3), <i>SYNJ2</i> (synaptojanin 2), IFI16 (interferon, gamma-inducible protein 16), <i>VEGFA</i> (vascular endothelial growth factor), <i>GFRA2</i> (GDNF family receptor alpha 2), and <i>FN1</i> (Fibronectin 1).