Phylogenetic tree of AphA orthologs.

<p>The protein sequences were derived from V. parahaemolyticus RIMD 2210633 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Makino1" target="_blank">[14]</a>, Vibrio sp. Ex25 (Accession numbers CP001805.1, and CP001806.1), Vibrio sp. EJY3 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Roh1" target="_blank">[48]</a>, V. harveyi ATCC BAA-1116 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Lin3" target="_blank">[49]</a>, V. vulnificus YJ016 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Chen1" target="_blank">[50]</a>, V. splendidus LGP32 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-LeRoux1" target="_blank">[51]</a>, V. anguillarum 775 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Naka1" target="_blank">[52]</a>, V. cholerae N16961 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Heidelberg1" target="_blank">[53]</a>, V. furnissii NCTC 11218 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Lux1" target="_blank">[54]</a>, and P. profundum SS9 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Vezzi1" target="_blank">[25]</a>. The a.a. sequences were aligned by the CLUSTALW <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Thompson1" target="_blank">[55]</a> web server at <a href="http://align.genome.jp/" target="_blank">http://align.genome.jp/</a>. The aligned sequences were then used to construct an unrooted neighbor-joining tree using MEGA version 5.0 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044210#pone.0044210-Tamura1" target="_blank">[56]</a> with a bootstrap iteration number of 1000. Shown on branch points of phylogenic tree were bootstrap values (%).