Phylogenetic tree of OpaR and its orthologs.

<p>Protein sequences were derived from <i>V. alginolyticus</i> ZJ-51 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Chang1" target="_blank">[60]</a>, <i>V. parahaemolyticus</i> RIMD 2210633 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Makino1" target="_blank">[16]</a>, <i>V. harveyi</i> ATCC BAA-1116 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Lin3" target="_blank">[61]</a>, <i>V. vulnificus</i> YJ016 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Chen1" target="_blank">[62]</a>, <i>V. tubiashii</i> RE22 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Hasegawa1" target="_blank">[63]</a>, <i>V. anguillarum</i> 775 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Naka1" target="_blank">[64]</a>, <i>V. cholera</i> N16961 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Heidelberg1" target="_blank">[65]</a>, and <i>V. fischeri</i> MJ11 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Mandel1" target="_blank">[66]</a>. The a.a. sequences were aligned by the CLUSTALW <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Thompson1" target="_blank">[67]</a> web server at <a href="http://align.genome.jp/" target="_blank">http://align.genome.jp/</a>. The aligned sequences were then used to construct an unrooted neighbor-joining tree using the MEGA version 5.0 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034622#pone.0034622-Tamura1" target="_blank">[68]</a> with a bootstrap iteration number of 1000. Shown on the branch points of phylogenic tree were the bootstrap values (%).