Inverse correlation of soluble and aggregated mhtt fragments analyzed by <i>Hdh</i>Q150/− forebrain tissue.

<p>SEC-FRET profiles with the UV absorbance in the upper right corner for (A) 3 months and (B) 12 months heterozygote <i>Hdh Q150/−</i> forebrain tissue. (C) Quantification of soluble mhtt by 2B7-Tb and MW1-D2 TR-FRET detection in supernatant fraction and of insoluble mhtt by MW8-Tb and MW8-D2 detection in resuspended pellet fraction of <i>Hdh Q150</i>/− mice at 3, 8 and 12 months. Delta F values (see experimental procedure) are mean of 4 independent measurements ± SEM. Delta F values are adjusted to protein concentration and then normalized to wild-type. The t-test was used to assess significance (D) Quantification of SEC fractions of peak 1 and of peak 2 by 2B7-Tb and MW1-D2 TR-FRET taken from <i>Hdh Q150</i>/− mice at 3, 8 and 12 months. Delta F values (mean of 4 independent measurements ± SEM) are expressed as the maximal peak value; The t-test was used to assess significance.