Analysis of R6/2 and zQ175 whole brain lysates after SEC.
- Wolfgang Reindl (5630)
- Barbara Baldo (500513)
- Jana Schulz (4183195)
- Isabell Janack (6508679)
- Ilka Lindner (6508682)
- Markus Kleinschmidt (6508685)
- Yalda Sedaghat (185223)
- Christina Thiede (139375)
- Karsten Tillack (720423)
- Christina Schmidt (36805)
- Isabell Cardaun (1655848)
- Tom Schwagarus (6508688)
- Frank Herrmann (218163)
- Madlen Hotze (6508691)
- Georgina F. Osborne (488792)
- Simone Herrmann (6508694)
- Andreas Weiss (135692)
- Celina Zerbinatti (275425)
- Gillian P. Bates (135712)
- Jonathan Bard (58130)
- Ignacio Munoz-Sanjuan (112471)
- Douglas Macdonald (562225)
Publication date
March 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Whole brain homogenates from (A) a 12 week-old R6/2 (500 μL at 3 mg/mL) and (B) a 14 month-old hetQ175 mouse (500 μL at 5 mg/mL) were run on a Superose 6 SEC column. Elution fractions were subsequently analyzed in the 2B7 / MW1-ST assay for human mHTT and the aggregate assays MW8 / 4C9-ST and MW8 / MW8-ST. Background: signal generated by sample buffer. Curve shapes were smoothed. Confirmation of detected HTT species by Western blotting of individual elution fractions failed as samples were too dilute after SEC.</p
Add to ORKG