Analysis of <i>Hdh</i>Q150/− striatum.
- David Marcellin (135678)
- Dorothee Abramowski (135682)
- Douglas Young (108969)
- Jens Richter (135687)
- Andreas Weiss (135692)
- Audrey Marcel (135697)
- Julia Maassen (135701)
- Muriel Kauffmann (135703)
- Miriam Bibel (135706)
- Derya R. Shimshek (135709)
- Richard L. M. Faull (135711)
- Gillian P. Bates (135712)
- Rainer R. Kuhn (135715)
- P. Herman Van der Putten (135721)
- Peter Schmid (81036)
- Gregor P. Lotz (135726)
DOIAbstract
<p>(A) Overlapped SEC-FRET profiles at 3, 4, 6 and 8 months of <i>Hdh</i>Q150/− striatum samples. (B) Detection of htt full-length protein (FL) by Western blot analysis in fractions 35–37 (peaks 1) and of mhtt fragments in fractions 44–49 (peak 2) using either 2B7 or MW1 antibodies. (C). Detection of mhtt fragments by Western Blot analysis of fractions 44, 45, 46 of <i>Hdh</i>Q150/− striatum samples at 3 and 8 months (D) Quantification of the insoluble mhtt by TR-FRET MW8-Tb and MW8-D2 detection. (E) Quantification of the soluble mhtt by TR-FRET 2B7-Tb and MW8-D2 detection. (F) Detection of mhtt fragments by Western Blot analysis of soluble (supernatant, 100000 g) and insoluble (pellet) brain extracts of <i>Hdh</i>Q150/− mice at 3, 8 and 12...
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