Expression of pathological tau in superficial EC layers of EC-hTau mice.

<p>(A–D) No obvious change in expression of the hTau transgene was observed between 4 and 16 months of age. (E–H) Abnormal conformation of tau, detected with the MC-1 antibody, was observed in EC neuropil at 4 months of age. At 12 and 16 months, cell bodies were also positively stained by MC-1 (G,H). (I–L) Phosphorylation of tau at serine 202 was detected with the CP-13 antibody. Cell bodies were positively stained for CP-13 across all ages, but some more intensely labeled neurons were observed at the older ages. CP-13 positive neuropil labeling also increased by 12 months (K). (M–P) Phosphorylations of tau at serine residues 199, 202, and 205 were detected with the AT8 antibody. Cell bodies were positively stained for AT8 across all ages. AT8 staining of the neuropil was maximal at 12 months (O). (Q–T) Phosphorylations of tau at serine 396 and 404 were detected with the PHF1 antibody. Scattered PHF1-positive cell bodies were seen at all ages. The neuropil and neurons were stained more intensely at 12 and 16 months (S,T). Scale bar (T) = 50 µm.