Pathological tau in the DG of EC-hTau mice.

<p>(A–D) hTau was detected in PP terminals in the outer molecular layer of the DG with the HT7 antibody at all ages examined. However, darkly stained cells in the granule cell layer (GCL) were seen primarily at 12 and 16 months (C, D). (E–H) PP terminals stained positive for MC-1 at all ages. By 12 and 16 months, GC also stained with MC-1 (G,H). (I–L) Faint CP13-positive PP terminals (arrows in K,L) and many more GC were observed at 12 and 16 months of age. (M–P) AT8 staining of the PP and GC increased markedly at 12 months (O) and many more positive GC were seen at 16 months (P). (Q–T) PHF1 staining of GC and the neuropil was also increased at 12 and 16 months (S,T). Scale bar (T) = 50 µm.