Plaque-associated tau pathology in rTg9191 mice.

<p>Brain sections from rTg9191 mice of 24 months of age (B, F, J, N, R, V, Z), their age-matched non-transgenic littermates (A, E, I, M, Q, U, Y), 23-month-old Tg2576 mice (C, G, K, O, S, W, AA) and 15-month-old rTg4510 mice (D, H, L, P, T, X, AB) were stained with a variety of antibodies directed against pathological conformation- and phosphorylation-dependent epitopes of tau: AT8 (A-D), CP13 (E-H), PG5 (I-L), PHF-1 (M-P), Alz50 (Q-T), MC1 (U-X) and TG-3 (Y-AB). Representative photomicrographs showed that hyperphosphorylated and/or misfolded tau proteins accumulated (brown puncta) around dense-core plaques visualized using Congo red (pink). Neuronal staining (brown) in rTg4510 mice served as positive control. Scale bars: 20 μm. Images in the same row have the same magnification. Representative photomicrographs show tau pathology of female mice, and similar results were found in male mice.