<p>(A) Alkaline phosphatase (AP) staining. Seven LMF-iPS cell lines examined expressed high levels of AP, similar to D3 ES cells. (B) Immunofluorescence staining of the ES cell-specific markers Oct4 and stage-specific embryonic antigen 1 (SSEA1). Scale bars: 200 µm. (C) mRNA expression of ES cell-specific markers (Nanog, Tert, Zfp, Oct4, and Sox2) and exogenous genes (Kl4 and c-Myc). G3PDH was used as a loading control.</p
<p>(A) The three hiPSC lines with typical morphology for human pluripotent stem cells expressed alka...
<p>V240M-LQTS-3 iPS cell colony derived from patient NP0016 showing typical human ES cell-like morph...
<p>(A) Alkaline phosphatase (AP) staining. Red-colored areas indicate alkaline phosphatase activity....
<p>(A) Left: Three-gene mouse iPSCs were capable of forming colonies similar in appearance to that o...
<p>(A, B) Phase contrast image and alkaline phosphatase staining of BALB/c ES cells in 1000 U/ml LIF...
<p>Unlike MEF (A-D), ES cells had alkaline phosphatase activity (E) and expressed SOX2 (F), Nanog2 (...
<p>(A and B) Quantification of mouse LIF and ActA protein concentrations in the culture media of SNL...
<p><b>A–B</b>. Undifferentiated iPS cells express and co-localize transcription factors Pou5f1 and N...
<p>Phase-contrast micrographs of colony morphology and alkaline phosphatase activity staining for mE...
<p>(A) Phase-contrast images of the two LMF-iPS cell lines, according to spontaneous differentiation...
<p>(A) Quantitative RT-PCR analysis of genes that are typically expressed in mouse naïve (<i>REX1</i...
-1 (A) and alkaline phosphatase (B). The expression of all markers was restricted to ES cells. Mouse...
<p>(A) Schematic representation of protocol for haematopoietic differentiation of X-CGD mouse derive...
<p>(A–J) iPSCs generated from DP264 grown in MSCGM-CD (D264-CD-iPS-2) and MSCGM (D264-M-iPS-5) expre...
<p>(A) Gene expression of previously reported mDA neuronal specific markers was confirmed by quantit...
<p>(A) The three hiPSC lines with typical morphology for human pluripotent stem cells expressed alka...
<p>V240M-LQTS-3 iPS cell colony derived from patient NP0016 showing typical human ES cell-like morph...
<p>(A) Alkaline phosphatase (AP) staining. Red-colored areas indicate alkaline phosphatase activity....
<p>(A) Left: Three-gene mouse iPSCs were capable of forming colonies similar in appearance to that o...
<p>(A, B) Phase contrast image and alkaline phosphatase staining of BALB/c ES cells in 1000 U/ml LIF...
<p>Unlike MEF (A-D), ES cells had alkaline phosphatase activity (E) and expressed SOX2 (F), Nanog2 (...
<p>(A and B) Quantification of mouse LIF and ActA protein concentrations in the culture media of SNL...
<p><b>A–B</b>. Undifferentiated iPS cells express and co-localize transcription factors Pou5f1 and N...
<p>Phase-contrast micrographs of colony morphology and alkaline phosphatase activity staining for mE...
<p>(A) Phase-contrast images of the two LMF-iPS cell lines, according to spontaneous differentiation...
<p>(A) Quantitative RT-PCR analysis of genes that are typically expressed in mouse naïve (<i>REX1</i...
-1 (A) and alkaline phosphatase (B). The expression of all markers was restricted to ES cells. Mouse...
<p>(A) Schematic representation of protocol for haematopoietic differentiation of X-CGD mouse derive...
<p>(A–J) iPSCs generated from DP264 grown in MSCGM-CD (D264-CD-iPS-2) and MSCGM (D264-M-iPS-5) expre...
<p>(A) Gene expression of previously reported mDA neuronal specific markers was confirmed by quantit...
<p>(A) The three hiPSC lines with typical morphology for human pluripotent stem cells expressed alka...
<p>V240M-LQTS-3 iPS cell colony derived from patient NP0016 showing typical human ES cell-like morph...
<p>(A) Alkaline phosphatase (AP) staining. Red-colored areas indicate alkaline phosphatase activity....