Characterization of stem cell properties of genetically modified, retrovirally transduced mESCs.
- Sungpil Cho (816561)
- Hongsuk Park (816562)
- Elke A. Jarboe (816563)
- C. Matthew Peterson (816564)
- You Han Bae (816565)
- Margit M. Janát-Amsbury (816566)
Publication date
October 2015
DOIAbstract
<p>(A) Alkaline phosphatase (AP) staining. Red-colored areas indicate alkaline phosphatase activity. (a). Bright field image of mESCs; AP staining: (b). mESCs; (c). mESC-H<i>ras</i>V12; (d). mESC-SV40 LTg; (e). mESC-H<i>ras</i>V12/SV40 LTg. Scale bare is 10 μm. (B) Immunocytochemistry staining of live-cells expressing SSEA1. Pairs of bright filed and SSEA1 images: (a). mESCs; (b). mESC-H<i>ras</i>V12; (c).mESC-SV40 LTg; (d). mESC-H<i>ras</i>V12/SV40 LTg. The representative images were obtained from fluorescent microscope with TRITC filter. Scale bar is 100 μm.</p
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