Cancer cell reprogramming conditions.
- Hisataka Ogawa (738260)
- Xin Wu (10164)
- Koichi Kawamoto (738261)
- Naohiro Nishida (503603)
- Masamitsu Konno (738262)
- Jun Koseki (738263)
- Hidetoshi Matsui (738264)
- Kozou Noguchi (738265)
- Noriko Gotoh (133877)
- Tsuyoshi Yamamoto (738266)
- Kanjiro Miyata (738267)
- Nobuhiro Nishiyama (338219)
- Hiroaki Nagano (282467)
- Hirofumi Yamamoto (1614)
- Satoshi Obika (738268)
- Kazunori Kataoka (281782)
- Yuichiro Doki (402704)
- Masaki Mori (30875)
- Hideshi Ishii (30870)
Publication date
May 2015
DOIAbstract
<p>Cells positive for the live stain alkaline phosphatase (AP) cells were counted as indicators of cancer reprogramming. HT29 cells were transfected with miRs as indicated and grown in culture under several conditions (culture medium, MEF feeder cells, and inhibitors). On day 21, AP staining was performed to evaluate reprogramming efficiency. Representative images are shown. Scale bars, 200 μm.</p
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